| 名称 |
anti- CASP3 antibody |
| 来源 |
Mouse |
| 应用 |
ELISA, IHC, WB,IF |
| 反应 |
Human, Mouse, Rat |
| 稀释范围 |
WB: 1:500-1:2000; IHC: 1:50-1:300; IF: 1:50-1:200 |
| 克隆 |
monoclonal |
| 蛋白号 |
P42574 |
| 分子质量 |
32-35 kDa, 19 kDa, 17 kDa |
| 纯化 |
protein A+G purified |
| 纯度 |
≥95% as determined by SDS-PAGE |
| 状态 |
liquid |
| 免疫原 |
caspase 3, apoptosis-related cysteine peptidase |
| 亚型 |
IgG1 |
| 基因ID |
2G7 |
| 存储 |
0.01MPBS pH 7.4,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| 别名 |
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1 |
| 背景介绍 |
Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3. |
