HCC70 (人乳腺导管癌细胞) (STR鉴定正确)

产品编号 HYPC2894
产品分类 细胞系(株)
品牌 华韵生物
规格 1×10⁶cells/T25培养瓶
品牌 货号 纯度 规格 目录价(CNY) 会员价(CNY) 数量 购物车
华韵生物 HYPC2894 1×10⁶cells/T25培养瓶 ¥6250.00
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名称 HCC70 (人乳腺导管癌细胞) (STR鉴定正确)
别称 HCC-70; HCC 70; HCC0070; Hamon Cancer Center 70
种属
生长特性 贴壁细胞
细胞形态 上皮细胞样
生长培养基 RPMI-164010% FBS1% P/S
冻存条件 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮
培养条件 气相:空气,95%;CO2,5% 温度:37℃
推荐传代比例 1:4 -1:6
推荐换液频率 2-3次/周
参考资料(来源文献)
背景描述 HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
年龄(性别) 女性,49岁
组织来源 乳腺
细胞类型 肿瘤细胞
肿瘤类型 乳腺导管癌
生物安全等级 1
倍增时间 93 hours (PubMed=25984343)
基因表达情况 epithelial glycoprotein 2 (EGP2); cytokeratin 19
保藏机构 ATCC; CRL-2315
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